Coding

Part:BBa_K4354012:Design

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-29)


ho1- pcyA gene to express a protein PCB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 749
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 749
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 759
    Illegal BglII site found at 879
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 749
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 749
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

According to the literature, we make an optimization to its sequence.We clone it to the vector pUC19 and test it by qRT-PCR.The stability of gene's expression is improved.

Source

We synthesize it according to its sequence and test it by DNA-sequencing.

References

1.Sebastian M. Castillo-Hair, Elliot A. Baerman, Masaya Fujita et al. Optogenetic control of Bacillus subtilis gene expression. NATURE COMMUNICATIONS doi.org/10.1038/s41467-019-10906-6.